9/4/2023 0 Comments Xa inhibitor reversal![]() Substantial evidence suggests that, as key cells involved in the formation of synovial tissue, fbroblast-like synoviocytes (FLSs) plays an important role in maintaining and exacerbating joint inflammation. The basic pathological changes are chronic inflammation of the joint synovium, the formation of pannus, and the gradual destruction of articular cartilage and bone, eventually leading to joint deformity and loss of function. Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease with erosive, symmetrical polyarthritis as the main clinical manifestation. In summary, circ_0000479 binds to miR-766 to promote RA progression via FKBP5. In addition, FKBP5 overexpression abated the inhibition of RA-FLS progression caused by circ_0000479 silencing. The results of dual-luciferase reporter assays also verified that FKBP5 was the target gene of miR-766. By intersecting the target genes of miR-766 with the up-regulated genes in RA, we obtained 8 genes, of which FKBP5 was most significantly down-regulated after miR-766 overexpression. Moreover, the inhibitory effect of circ_0000479 knockdown in RA-FLS progression was attenuated by miR-766 inhibitor. MiR-766 was confirmed to be down-regulated in RA-FLSs and the combination between circ_0000479 and miR-766 was verified by dual-luciferase reporter assays. Through bioinformatics analysis and screening, we obtained 18 miRNAs that can bind to circ_0000479, of which miR-766 was most significantly up-regulated after circ_0000479 knockdown. The results confirmed that circ_0000479 knockdown suppressed pathogenic properties of RA-FLSs. Flow cytometry analysis, cell counting Kit-8, transwell, wound-healing and enzyme-linked immunosorbent assays were conducted to evaluate RA-FLS apoptosis, proliferation, invasion, migration and inflammation. Circ_0000479 was found to be upregulated in RA-FLSs. This study aimed to investigate the function of circ_0000479 in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). ![]() Circular RNAs have been demonstrated to play a critical role in the progression of autoimmune diseases. ![]()
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